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SWIM-DOWN TECHNIQUE

Single Density Gradient Media
i)   CRYO-1 2ml - 50% density gradient
    5mi- HTF wash media
ii)   CRYO-1 HD 2ml-80% density gradient
    5mi- HTF wash media
iii)   CRYO-2 1ml- 45% density gradient
    1 ml-90% density gradient
    5ml- HTF wash media
iv)   SOP CRYO-1
    CRYO-2